mouse anti ep fitc  (Cedarlane)

Journal: iScience

Article Title: Hypoxia stimulates CTC-platelet cluster formation to promote breast cancer metastasis.

doi: 10.1016/j.isci.2024.109547

Figure Lengend Snippet: Figure 4. Specific genes related to hypoxia stimulation and platelet activation were enriched in the CTM group versus the control group (A) Images of CTM released from the Cluster-Chip and live stained with FITC anti-mouse CD326 (EPCAM) (green) and Alex594-conjugated antibodies against CD45 (red). (B) Heatmap of gene expression related to epithelial status, mesenchymal status, cell stemness, leukocytes, cell junctions, and platelets in samples from each group. (C) Gene Ontology enrichment analysis in treatment vs. control groups. (D) Heatmap of gene expression associated with CTM-mediating metastasis in the two groups of samples, with some genes marked with red and some marked with green relating to hypoxia stimulation and platelet activation, respectively. (E) Verification of key genes that are differently expressed in treatment vs. control group by qPCR. (F) Representative fluorescent micrographs of 4T1 cell clusters captured on the chip following culture in ultra-low adhesion well plates with or without platelets under the hypoxic or normoxic conditions. CTC-clusters stained for Pan-cytokeratin and EPCAM (green), P-selectin (red), and DAPI (nuclei, blue). Scale bar: 50 mm and 20 mm. (G) The statistic quantification of the different types of captured CTM cultured in 4 conditions as mentioned in (F). Data were presented as mean G SD. *0.01 < p % 0.05; **0.001 < p % 0.01; ***p % 0.001.

Article Snippet: Antibodies Anti-pan cytokeratin Abcam ab7753 P-Selectin polyclonal antibody Proteintech 13304-1-AP CD45R (RA3-6B2) Santa Cruz sc-19597 Alexa Fluor 488-labeled goat anti-mouse lgG (H + L) Proteintech SA00013-1 594-labeled goat anti-rabbit lgG (H + L) Proteintech SA00013-4 TRITIC-labeled goat anti-Rat lgG (H + L) Proteintech SA00007-7 CD45 Rabbit Polyclonal antibody Proteintech 20103-1-AP FITC anti-mouse CD326 (Ep-CAM) Biolegend 118207 Alex 594 anti-mouse CD45 Biolegend 103144 Chemicals, peptides, and recombinant proteins Fetal bovine serum ExCell Bio FSP500 RPMI-1640 medium HyClone SH30027.02

Techniques: Activation Assay, Control, Staining, Gene Expression, Cell Culture

Journal: Cell Reports

Article Title: Trypanosoma brucei ATR Links DNA Damage Signaling during Antigenic Variation with Regulation of RNA Polymerase I-Transcribed Surface Antigens

doi: 10.1016/j.celrep.2019.12.049

Figure Lengend Snippet:

Article Snippet: Anti-EP Procyclic FITC Conjugate , Cederlane , Cat#CLP001F.

Techniques: Virus, Recombinant, SYBR Green Assay, Sequencing, Plasmid Preparation, Software